Difference between revisions of "Part:BBa K3792004"
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<partinfo>BBa_K3792003 short</partinfo>
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apFAB51 is a strong promoter taken from the BIOFAB collection. This device uses apFAB51 to express mRFP1, a red fluorescent protein. The apFAB51 test device consists of apFAB51, an RBS, the coding sequence for mRFP1, and a terminator.
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apFAB98 is a weak promoter taken from the BIOFAB collection.  
  
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This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.  
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This device uses apFAB98 to express mRFP1, a red fluorescent protein. The apFAB98 test device consists of apFAB98, an RBS, the coding sequence for mRFP1, and a terminator.
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This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.
  
 
The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.
 
The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3792004 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3792003 parameters</partinfo>
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<partinfo>BBa_K3792004 parameters</partinfo>
 
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Revision as of 00:38, 20 October 2021

BIOFAB apFAB98 Measurement Device

apFAB98 is a weak promoter taken from the BIOFAB collection.

This device uses apFAB98 to express mRFP1, a red fluorescent protein. The apFAB98 test device consists of apFAB98, an RBS, the coding sequence for mRFP1, and a terminator.

This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.

The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 615
    Illegal AgeI site found at 727
  • 1000
    COMPATIBLE WITH RFC[1000]