Difference between revisions of "Part:BBa K3792006"
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apFAB98 is a weak promoter taken from the BIOFAB collection. This device uses apFAB98 to express mRFP1, a red fluorescent protein. The apFAB98 test device consists of apFAB98, an RBS, the coding sequence for mRFP1, and a terminator.
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This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.
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The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.
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The NodCIJ Generator with Weak Promoter does not produce a lot of the NodC protein, NodI protein, and NodJ protein. The NodC protein produces chitin and NodI and NodJ transports nodulation factors.
 
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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This part consists of 2555 base-pair length. It includes a promoter, 3 ribosome binding sites, 3 protein-coding sequences, and a terminator.
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3792006 parameters</partinfo>
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<partinfo>BBa_K3792003 parameters</partinfo>
 
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Revision as of 00:32, 20 October 2021

BIOFAB apFAB40 Measurement Device

apFAB98 is a weak promoter taken from the BIOFAB collection. This device uses apFAB98 to express mRFP1, a red fluorescent protein. The apFAB98 test device consists of apFAB98, an RBS, the coding sequence for mRFP1, and a terminator.

This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.

The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
    Illegal AgeI site found at 726
  • 1000
    COMPATIBLE WITH RFC[1000]