Difference between revisions of "Part:BBa K3726051"

 
 
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__NOTOC__
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<partinfo>BBa_K3726051 short</partinfo>
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This is a composite part that corresponds with a level 1 MoClo part. It is a transcriptional unit for the expression of the part “BBa_K3726018” CDS_Lv0_BOH_1_C_FRRRF , the last two enzymes of synthetic butanol biosynthesis operon linked with a flexible and rigid tandem without including a stop codon between themselves.
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The part in its upstream region, has the connector 5'Con3
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“K2560067” that will allow the assembly of a Lv2 construct following the marburg collection standard. In its downstream region has a homology region
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“BBa_K3726105” 3CON5(H)_NS1(mod)-down (PCC 11801) for homologous recombination within the genome of PCC 11801.
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Likewise, a Kanamycin resistance cassette is included downstream the transcriptional unit region.
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Expression is controlled by the medium-strong synthetic constitutive promoter J23119 “BBa_K2560031”and by the strong synthetic RBS, RBS_PduP “BBa_K3726090”.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3726051 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K3726051 parameters</partinfo>
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Latest revision as of 00:03, 20 October 2021


L1_BOH1C-FRRRF

This is a composite part that corresponds with a level 1 MoClo part. It is a transcriptional unit for the expression of the part “BBa_K3726018” CDS_Lv0_BOH_1_C_FRRRF , the last two enzymes of synthetic butanol biosynthesis operon linked with a flexible and rigid tandem without including a stop codon between themselves.

The part in its upstream region, has the connector 5'Con3 “K2560067” that will allow the assembly of a Lv2 construct following the marburg collection standard. In its downstream region has a homology region “BBa_K3726105” 3CON5(H)_NS1(mod)-down (PCC 11801) for homologous recombination within the genome of PCC 11801.

Likewise, a Kanamycin resistance cassette is included downstream the transcriptional unit region.

Expression is controlled by the medium-strong synthetic constitutive promoter J23119 “BBa_K2560031”and by the strong synthetic RBS, RBS_PduP “BBa_K3726090”.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1751
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 431
    Illegal NheI site found at 454
    Illegal NheI site found at 757
    Illegal NheI site found at 985
    Illegal PstI site found at 1751
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2146
    Illegal BglII site found at 2263
    Illegal BglII site found at 4682
    Illegal XhoI site found at 3366
    Illegal XhoI site found at 4209
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1751
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1751
  • 1000
    COMPATIBLE WITH RFC[1000]