Difference between revisions of "Part:BBa K3726049:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K3726049 short</partinfo>
  
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<partinfo>BBa_K3726049 SequenceAndFeatures</partinfo>
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===Design Notes===
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The part is a MoClo Lv.1 part, assembled in the acceptor Lv1/2 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” MARK_Lv0_AmpR.
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This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the parts “BBa_K3726104” 5CON1(H)_NS1(mod)-up (PCC 11801) and “BBa_K3726105 ” 3CON5(H)_NS1(mod)-down (PCC 11801) allowing the Integration within the PCC 11801 Genome.
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===Source===
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This construct has been made by golden gate reaction.
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===References===
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X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.

Latest revision as of 23:46, 19 October 2021

BOH3_LV1 (A)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1786
    Illegal PstI site found at 18
    Illegal PstI site found at 1221
    Illegal PstI site found at 1691
    Illegal PstI site found at 1939
    Illegal PstI site found at 3240
    Illegal PstI site found at 6255
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1786
    Illegal NheI site found at 5261
    Illegal NheI site found at 5489
    Illegal PstI site found at 18
    Illegal PstI site found at 1221
    Illegal PstI site found at 1691
    Illegal PstI site found at 1939
    Illegal PstI site found at 3240
    Illegal PstI site found at 6255
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1786
    Illegal BglII site found at 6600
    Illegal BglII site found at 6717
    Illegal BglII site found at 9136
    Illegal BamHI site found at 3323
    Illegal BamHI site found at 3662
    Illegal XhoI site found at 4595
    Illegal XhoI site found at 7820
    Illegal XhoI site found at 8663
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1786
    Illegal PstI site found at 18
    Illegal PstI site found at 1221
    Illegal PstI site found at 1691
    Illegal PstI site found at 1939
    Illegal PstI site found at 3240
    Illegal PstI site found at 6255
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1786
    Illegal PstI site found at 18
    Illegal PstI site found at 1221
    Illegal PstI site found at 1691
    Illegal PstI site found at 1939
    Illegal PstI site found at 3240
    Illegal PstI site found at 6255
    Illegal NgoMIV site found at 1430
    Illegal NgoMIV site found at 1530
    Illegal NgoMIV site found at 1836
    Illegal NgoMIV site found at 3918
    Illegal AgeI site found at 594
    Illegal AgeI site found at 2208
    Illegal AgeI site found at 3446
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a MoClo Lv.1 part, assembled in the acceptor Lv1/2 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” MARK_Lv0_AmpR. This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the parts “BBa_K3726104” 5CON1(H)_NS1(mod)-up (PCC 11801) and “BBa_K3726105 ” 3CON5(H)_NS1(mod)-down (PCC 11801) allowing the Integration within the PCC 11801 Genome.

Source

This construct has been made by golden gate reaction.

References

X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.