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Revision as of 23:42, 19 October 2021


Trace and Control System

Trace and Control System was used in Hidro Project by NDNF_China 2020.

Characterization

To dynamically monitor and control the engineered bacteria beyond the laboratory, we added DNA barcode system and kill switch to microbial genome through gene editing. The genetic circuits design is shown in the following diagram (Figure 1A & 1B). The genome-integrated Tracing and Control system offers tracking and specific killing of engineered strains in case of emergencies.

Figure 1: (A)The design scheme of Trace and Control System in Hidro; (B) The gel image of the result of genome integration of Trace and Control System into fimA site.

Tracing: Customizable Barcode and CRISPR-Cas12a nucleic acid testing offers the way to efficiently tracing engineered bacteria

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1269
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1521
    Illegal AgeI site found at 1104
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1086