Difference between revisions of "Part:BBa K3286138"
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Felix, G., Duran, J. D., Volko, S., & Boller, T. (1999). Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. The Plant Journal : For Cell and Molecular Biology, 18(3), 265–276. https://doi.org/10.1046/j.1365-313x.1999.00265.x
 
Felix, G., Duran, J. D., Volko, S., & Boller, T. (1999). Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. The Plant Journal : For Cell and Molecular Biology, 18(3), 265–276. https://doi.org/10.1046/j.1365-313x.1999.00265.x
 
Felix, G., Duran, J. D., Volko, S., & Boller, T. (1999). Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. The Plant Journal : For Cell and Molecular Biology, 18(3), 265–276. https://doi.org/10.1046/j.1365-313x.1999.00265.x
 
Felix, G., Duran, J. D., Volko, S., & Boller, T. (1999). Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. The Plant Journal : For Cell and Molecular Biology, 18(3), 265–276. https://doi.org/10.1046/j.1365-313x.1999.00265.x
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===Characterization by team 2021 UZurich===
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[[File:flg22 vs elf18.jpg|500px]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:03, 19 October 2021


flg22

Flg22 is a peptide consisting of 22 conserved amino acids at the N-terminus flaggelin of Pseudomonas syringae [Felix et al., 1999]. Flg22 is a well described Microbe Associated Molecular Pattern (MAMPs). If recognised, MAMPS trigger PAMP Triggered Immunity in plants. They have been shown to trigger disease resistance in plants. [Zipfel et al., 2004]. flg22 can be used to active an immune response in plants.

It is very hard to purify this peptide if recombinantly expressed in E. coli, due to the small size and the folding of this peptide.

Felix, G., Duran, J. D., Volko, S., & Boller, T. (1999). Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. The Plant Journal : For Cell and Molecular Biology, 18(3), 265–276. https://doi.org/10.1046/j.1365-313x.1999.00265.x Felix, G., Duran, J. D., Volko, S., & Boller, T. (1999). Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. The Plant Journal : For Cell and Molecular Biology, 18(3), 265–276. https://doi.org/10.1046/j.1365-313x.1999.00265.x

Characterization by team 2021 UZurich

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 58
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 58
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 58
    Illegal NgoMIV site found at 52
    Illegal AgeI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]