Difference between revisions of "Part:BBa K3726045:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K3726045 short</partinfo>
  
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<partinfo>BBa_K3726045 SequenceAndFeatures</partinfo>
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===Design Notes===
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The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR).  This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by two connectors that will allow  the assembly of a Lv2 construct. In its upstream region the connector is 5'Con2 BBa_K2560066, and in its downstream region 3'Con2 BBa_K2560071.
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===Source===
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This construct has been made by golden gate reaction.
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===References===
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X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.

Latest revision as of 22:13, 19 October 2021

BOH1_B_LV1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 654
    Illegal PstI site found at 903
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 654
    Illegal PstI site found at 903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 986
    Illegal BamHI site found at 1325
    Illegal XhoI site found at 2234
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 654
    Illegal PstI site found at 903
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 654
    Illegal PstI site found at 903
    Illegal NgoMIV site found at 1581
    Illegal AgeI site found at 109
    Illegal AgeI site found at 1109
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by two connectors that will allow the assembly of a Lv2 construct. In its upstream region the connector is 5'Con2 BBa_K2560066, and in its downstream region 3'Con2 BBa_K2560071.

Source

This construct has been made by golden gate reaction.

References

X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.