Difference between revisions of "Part:BBa K3726044:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K3726044 short</partinfo>
  
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<partinfo>BBa_K3726044 SequenceAndFeatures</partinfo>
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===Design Notes===
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The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR).  This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the part “BBa_K3726104”  (5CON1(H)_NS1(mod)-up (PCC 11801)) that allows homologous recombination within the PCC 11801 Genome, and by the part  3'Con1 “BBa_K2560070” that will allow  the assembly of a Lv2 construct.
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===Source===
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This construct has been made by golden gate reaction.
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===References===
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X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.

Latest revision as of 22:00, 19 October 2021

BOH_1_A_LV1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal PstI site found at 18
    Illegal PstI site found at 1755
    Illegal PstI site found at 2003
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal PstI site found at 18
    Illegal PstI site found at 1755
    Illegal PstI site found at 2003
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal PstI site found at 18
    Illegal PstI site found at 1755
    Illegal PstI site found at 2003
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal PstI site found at 18
    Illegal PstI site found at 1755
    Illegal PstI site found at 2003
    Illegal NgoMIV site found at 1494
    Illegal NgoMIV site found at 1594
    Illegal NgoMIV site found at 1900
    Illegal AgeI site found at 594
    Illegal AgeI site found at 2272
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the part “BBa_K3726104” (5CON1(H)_NS1(mod)-up (PCC 11801)) that allows homologous recombination within the PCC 11801 Genome, and by the part 3'Con1 “BBa_K2560070” that will allow the assembly of a Lv2 construct.

Source

This construct has been made by golden gate reaction.

References

X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.