Difference between revisions of "Part:BBa K3771042"

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	The his-tag allows for confirmation of CDO1 expression by western blot using the anti-6X his-tag antibody.<br><br><b style="font-size:1.3rem">Characterization</b><br>		The his-tag allows for confirmation of CDO1 expression by western blot using the anti-6X his-tag antibody.<br><br><b style="font-size:1.3rem">Characterization</b><br>
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−	<img src="https://2021.igem.org/wiki/images/c/c0/T--NCKU_Tainan--colony_pspAcs.jpg" style="width:40%;">	+	<img src="https://2021.igem.org/wiki/images/f/fb/T--NCKU_Tainan--colony_pspAcdo1.jpg" style="width:40%;">
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	<p align="center">Fig. 2. Colony PCR confirmation of the construction</p>		<p align="center">Fig. 2. Colony PCR confirmation of the construction</p>

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Revision as of 21:59, 19 October 2021

PpspA-CDO1-6xHis

Description

This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CDO1.

Biology

Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [1] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CDO1-his-tag. CDO1 converts L-cysteine into L-cysteine sulfonic acid in the taurine synthesis L-cysteine sulfinic acid pathway [2].

Fig.1 Taurine pathways in E. coli

Usage

We ligased the cdo1-6xHis fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. The his-tag allows for confirmation of CDO1 expression by western blot using the anti-6X his-tag antibody.

Characterization

Fig. 2. Colony PCR confirmation of the construction

Reference

1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.xhttps://pubmed.ncbi.nlm.nih.gov/16045608/

2. Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093https://pubmed.ncbi.nlm.nih.gov/30516051/
Sequence and Features BBa_K3771042 SequenceAndFeatures