Difference between revisions of "Part:BBa K3771024"
Line 4: | Line 4: | ||
<html> | <html> | ||
− | <br><b style="font-size:1.3rem">Description</b> | + | <br><b style="font-size:1.3rem">Description</b><br> |
<br>This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CSAD. | <br>This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CSAD. | ||
<br> | <br> | ||
− | <br><b style="font-size:1.3rem">Biology</b> | + | <br><b style="font-size:1.3rem">Biology</b><br> |
− | <br>Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. <a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">[ | + | <br>Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. <a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">[1]</a> Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CSAD. CSAD catalyzes the decarboxylation of L-Cysteine sulfinic acid into hypotaurine, which is spontaneously oxidized to taurine <a href="https://pubmed.ncbi.nlm.nih.gov/30516051/" alt="" target="_blank">[4]</a>. <br> |
− | <br><b style="font-size:1.3rem">Usage</b> | + | <br><b style="font-size:1.3rem">Usage</b><br> |
<br>We ligased the <i>csad</i> fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. | <br>We ligased the <i>csad</i> fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. | ||
<br> | <br> | ||
− | <br><b style="font-size:1.3rem">Characterization</b> | + | <br><b style="font-size:1.3rem">Characterization</b><br> |
<div style="width=100%; display:flex; align-items: center; justify-content: center;"> | <div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
<img src="https://2021.igem.org/wiki/images/1/13/T--NCKU_Tainan--colony_pcr_pspA_CSAD.jpg" style="width:35%;"> | <img src="https://2021.igem.org/wiki/images/1/13/T--NCKU_Tainan--colony_pcr_pspA_CSAD.jpg" style="width:35%;"> | ||
Line 18: | Line 18: | ||
<p align="center">圖片描述</p> | <p align="center">圖片描述</p> | ||
− | <br><b style="font-size:1.3rem">Reference</b> | + | <br><b style="font-size:1.3rem">Reference</b><br> |
− | <br> | + | <br>1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/ |
" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/ | " alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/ | ||
</a><br> | </a><br> | ||
− | <br> | + | <br>2. Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093<a href="https://pubmed.ncbi.nlm.nih.gov/30516051/" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/30516051/</a><br> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 20:26, 19 October 2021
PpspA-CSAD
Description
This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CSAD.
Biology
Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [1] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CSAD. CSAD catalyzes the decarboxylation of L-Cysteine sulfinic acid into hypotaurine, which is spontaneously oxidized to taurine [4].
Usage
We ligased the csad fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.
Characterization
![](https://2021.igem.org/wiki/images/1/13/T--NCKU_Tainan--colony_pcr_pspA_CSAD.jpg)
圖片描述
Reference
1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.xhttps://pubmed.ncbi.nlm.nih.gov/16045608/
2. Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093https://pubmed.ncbi.nlm.nih.gov/30516051/
Sequence and Features