Difference between revisions of "Part:BBa K3794005"
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This composite part can be utilised with any RFC[10] compatible plasmid backbone. | This composite part can be utilised with any RFC[10] compatible plasmid backbone. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3794005 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3794005 SequenceAndFeatures</partinfo> | ||
Revision as of 20:22, 19 October 2021
ChABC Composite
This composite part acts as a translational unit for the expression of ChABC in E.coli. The LacI regulatory system (BBa_R0010) which encodes the lac promoter and operator allow for IPTG-induced expression in E.coli. BBa_B0010, a T1 terminator from E.coli rrnB also allows for termination of transcription. As the ChABC basic part encodes a 6xHis tag and TEV cleavage site, protein purification using a Ni-NTA column can be achieved.
This composite part can be utilised with any RFC[10] compatible plasmid backbone.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2954
- 1000COMPATIBLE WITH RFC[1000]