Difference between revisions of "Part:BBa K3794005"

 
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<partinfo>BBa_K3794005 short</partinfo>
 
<partinfo>BBa_K3794005 short</partinfo>
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This composite part acts as a translational unit for the expression of ChABC in E.coli. The LacI regulatory system (BBa_R0010) which encodes the <i> lac </i> promoter and operator allow for IPTG-induced expression in E.coli. BBa_B0010, a T1 terminator from rrnB also allows for termination of transcription. As the ChABC basic part encodes a 6xHis tag and TEV cleavage site, protein purification using a Ni-NTA column can be achieved.
  
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Expression vector for ChABC using pSB1A3 plasmid
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This composite part can be utilised with any RFC[10] compatible plasmid backbone.
  
 
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Revision as of 20:18, 19 October 2021


ChABC Composite This composite part acts as a translational unit for the expression of ChABC in E.coli. The LacI regulatory system (BBa_R0010) which encodes the lac promoter and operator allow for IPTG-induced expression in E.coli. BBa_B0010, a T1 terminator from rrnB also allows for termination of transcription. As the ChABC basic part encodes a 6xHis tag and TEV cleavage site, protein purification using a Ni-NTA column can be achieved.

This composite part can be utilised with any RFC[10] compatible plasmid backbone.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2954
  • 1000
    COMPATIBLE WITH RFC[1000]