Difference between revisions of "Part:BBa K1159105:Experience"

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===User Reviews===
 
===User Reviews===
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'''Sao Carlos-Brazil'''
 
The Sao Carlos-Brazil Team has developed a codon-optimized version of this part, it can be accessed at https://parts.igem.org/Part:BBa_K3273013
 
The Sao Carlos-Brazil Team has developed a codon-optimized version of this part, it can be accessed at https://parts.igem.org/Part:BBa_K3273013
  
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'''NUS Singapore 2021'''
 
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Attempted to perform part improvement by adding a nuclear localization signal tag to the 5' end of the part sequence to improve the kill switch efficacy in part <partinfo>Ba_K3927015</partinfo>.
 
Attempted to perform part improvement by adding a nuclear localization signal tag to the 5' end of the part sequence to improve the kill switch efficacy in part <partinfo>Ba_K3927015</partinfo>.
  
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https://2021.igem.org/wiki/images/8/8a/T--NUS_Singapore--results_tab3_3.png
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<i> Figure 1: Construct schematic of part assembled with a Gal1 promoter upstream to trigger galactose dependent endonuclease activity</i>
 
<i> Figure 1: Construct schematic of part assembled with a Gal1 promoter upstream to trigger galactose dependent endonuclease activity</i>

Latest revision as of 19:49, 19 October 2021


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Please enter how you used this part and how it worked out.

Applications of BBa_K1159105

User Reviews

Sao Carlos-Brazil The Sao Carlos-Brazil Team has developed a codon-optimized version of this part, it can be accessed at https://parts.igem.org/Part:BBa_K3273013

NUS Singapore 2021

•••

CWChew

NUS Singapore 2021 has successfully cloned part into S. cerevisiae and tested part by placing nuclease downstream of the galactose inducible promoter Gal1 (Figure 1). Kill switch efficacy was not optimal and had a 82% survival rate.

Attempted to perform part improvement by adding a nuclear localization signal tag to the 5' end of the part sequence to improve the kill switch efficacy in part BBa_K3927015.

T--NUS_Singapore--results_tab3_3.png


Figure 1: Construct schematic of part assembled with a Gal1 promoter upstream to trigger galactose dependent endonuclease activity

T--NUS_Singapore--results_tab3_5.png

Figure 2: CFU assay results from pGNucA-H(BY4741) and pGNLSNucA-H(BY4741), ratio of colonies counted from plates with the nuclease induced to the colonies counted from the plates with nuclease uninduced. Nuclease with the NLS attached reduces the total number of live colonies on the plate.

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UNIQb0fbe3fb3d7e5250-partinfo-00000002-QINU UNIQb0fbe3fb3d7e5250-partinfo-00000003-QINU