Difference between revisions of "Part:BBa K3893030"

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=== Design ===
 
=== Design ===
 
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Module 2 was designed for delivering and releasing dsRNA molecules of the <i>velvet gene</i> produced by Module 1. However, we replaced Module 1 by a reporter to test the effectiveness of cell lysis. To this end, LuxR-AHL transcription factor activates simultaneously the expression of GFP and the lysis protein PhiX174E. Further details of the final design including its four transcriptional units can be found here.
 
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<img style="width: 80%" src="https://2021.igem.org/wiki/images/1/18/T--Ecuador--Eng_DBcircuit.png">
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3893030 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3893030 SequenceAndFeatures</partinfo>

Revision as of 19:27, 19 October 2021


Population control device (QS-based lysis protein oscilator)

domestication.
Figure 1. SBOL Visual representation of the device

This device is an AHL induced transcriptional unit to express phiX174 lysis protein together with a TU for the constitutive expression of LuxI. It was assembled with a one-pot Level 2 Golden Gate reaction using BsmBI type IIS endonuclease.

This device is composed of the following standardized composite parts:

  • BBa_K3893028: a Level 2 transcriptional unit constitutively expressing luxI gene (Golden Braid compatible)
  • BBa_K3893029: a Level 2 transcriptional unit expresing phiX174 lysis protein under the control of the pux promoter (Golden Braid compatible)


Usage and Biology

Part engineering and DBTL cycle

The development of this part was performed following the steps of the Design-Build-Test-Learn (DBTL) cycle of synthetic biology. Here, we describe the different steps and the results obtained.

Design

Module 2 was designed for delivering and releasing dsRNA molecules of the velvet gene produced by Module 1. However, we replaced Module 1 by a reporter to test the effectiveness of cell lysis. To this end, LuxR-AHL transcription factor activates simultaneously the expression of GFP and the lysis protein PhiX174E. Further details of the final design including its four transcriptional units can be found here.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 956
    Illegal NheI site found at 979
    Illegal NheI site found at 1914
    Illegal NheI site found at 1937
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]