Difference between revisions of "Part:BBa K3726039:Design"

 
 
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===Design Notes===
 
===Design Notes===
 
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As an antibiotic resistance part, the upstream overhang is GGTA, while the downstream overhang is CGCT, in accordance with the Marburg Collection design guidelines.  
 
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As an antibiotic resistance part, the upstream overhang is GGTA, while the downstream overhang is CGCT, in accordance with the Marburg Collection design guidelines.  
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===Source===
 
===Source===
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===References===
 
===References===
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Stukenberg D;Hensel T;Hoff J;Daniel B;Inckemann R;Tedeschi JN;Nousch F;Fritz G; The marburg collection: A golden gate DNA assembly framework for synthetic biology applications in vibrio Natriegens. ACS synthetic biology. Available at: https://pubmed.ncbi.nlm.nih.gov/34255476/ .

Latest revision as of 19:23, 19 October 2021


MARK_Lv0_AmpR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As an antibiotic resistance part, the upstream overhang is GGTA, while the downstream overhang is CGCT, in accordance with the Marburg Collection design guidelines.

Source

References

Stukenberg D;Hensel T;Hoff J;Daniel B;Inckemann R;Tedeschi JN;Nousch F;Fritz G; The marburg collection: A golden gate DNA assembly framework for synthetic biology applications in vibrio Natriegens. ACS synthetic biology. Available at: https://pubmed.ncbi.nlm.nih.gov/34255476/ .