Difference between revisions of "Part:BBa K3794001"

Revision as of 18:46, 19 October 2021

PVFP-5 Composite

This composite part is a translational unit for PVFP-5. It is used for expression of a 6xHis tagged PVFP-5 protein in E.coli. It is under the control of the lac promoter and operator. It also contains a CAP binding site. It has been codon optimised for expression in E.coli K12, and was used with pSB1A3 by KCL iGEM 2021.

Expression can be conducted using IPTG-induction, and the expressed protein can be purified using a Ni-NTA column.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3794001 short</partinfo>
-This part is the expression vector for expression of Pvfp-5 in E.Coli. It contains a lac promoter and operator, a codon-optimised gene insert for Pvfp-5, and an N-terminal 6xHis + TEV site. The plasmid used is pSB1A3.
+This composite part is a translational unit for PVFP-5. It is used for expression of a 6xHis tagged PVFP-5 protein in E.coli. It is under the control of the <i> lac </i> promoter and operator. It also contains a CAP binding site. It has been codon optimised for expression in E.coli K12, and was used with pSB1A3 by KCL iGEM 2021.
-This expression vector can be used to synthesise Pvfp-5 and allows protein purification through a Ni-NTA column.
+Expression can be conducted using IPTG-induction, and the expressed protein can be purified using a Ni-NTA column.
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