Difference between revisions of "Part:BBa M10210"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_M10210 short</partinfo>
 
<partinfo>BBa_M10210 short</partinfo>
  
 
Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a silver binding peptide {AG4}, circularly permutated ompG {CPG_L6} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}
 
Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a silver binding peptide {AG4}, circularly permutated ompG {CPG_L6} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}
 +
 +
[[Template:BioE140LSpr09-Streptavidin | Streptavidin binding assay template]]<br>
 +
[[Template:BioE140LSpr09-Growth | Growth curve assay template]]<br>
 +
[[Template:BioE140LSpr09-Silver | Silver binding assay template]]<br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:04, 10 May 2009

{Pbad.rbs.prepro.StrepTag}{AG4}{CPG_L6!}{dblTerm}

Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a silver binding peptide {AG4}, circularly permutated ompG {CPG_L6} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}

Streptavidin binding assay template
Growth curve assay template
Silver binding assay template

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1023
    Illegal AgeI site found at 2123
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1005