Difference between revisions of "Part:BBa K3861024"
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<partinfo>BBa_K3861024 short</partinfo> | <partinfo>BBa_K3861024 short</partinfo> | ||
| − | + | Plldr-sicP-spt-Pep8 | |
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| + | Composite part consisting of <a href="https://parts.igem.org/Part:BBa_K3861024?action=edit">BBa_K3861024</a> and <a href="https://parts.igem.org/Part:BBa_K3861010?action=edit">BBa_K3861010</a>. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediate by PlldR. Further, due to the SPI T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI T3SS. | ||
| + | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 18:30, 19 October 2021
PlldR-sicP-sptP-Pep8
Plldr-sicP-spt-Pep8 Composite part consisting of BBa_K3861024 and BBa_K3861010. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediate by PlldR. Further, due to the SPI T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI T3SS.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 519
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 78
Illegal NheI site found at 101
Illegal PstI site found at 519 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 519
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 519
- 1000COMPATIBLE WITH RFC[1000]