Difference between revisions of "Part:BBa K3733001"
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The histidine kinase is a part of two-component signal system, which each constitute a sensor histidine kinase and a response regulator. The NarX is sensor histidine kinase, which can phosphorylate NarL in response to nitrate and nitrite. | The histidine kinase is a part of two-component signal system, which each constitute a sensor histidine kinase and a response regulator. The NarX is sensor histidine kinase, which can phosphorylate NarL in response to nitrate and nitrite. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | <p>The NarX/NarL two component system(TCS) can be activated if the concentration of nitrate is enough. So this NarX/NarL TCS can be designed the nitrate sensor with the PyeaR promoter. When there is enough nitrate, the NarX will change configuration and then phosphorylate. After that, the phosphorylated NarX will transport the phosphate group to NarL. Last, the phosphorylated NarL will bind to the certain sequence of PyeaR, and it acts as a transcription factor, which can active the downstream of PyeaR to transcription.</p> | ||
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| + | <p>The NarX (C415R) is a mutant of NarX (BBa_K3733000), which amino acid at position of 415 changed from cysteine to arginine. For this change, we can make the nitrate sensing system more sensitive. </p> | ||
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Revision as of 17:28, 19 October 2021
NarX(C415R): A point mutation of NarX
The histidine kinase is a part of two-component signal system, which each constitute a sensor histidine kinase and a response regulator. The NarX is sensor histidine kinase, which can phosphorylate NarL in response to nitrate and nitrite.
Usage and Biology
The NarX/NarL two component system(TCS) can be activated if the concentration of nitrate is enough. So this NarX/NarL TCS can be designed the nitrate sensor with the PyeaR promoter. When there is enough nitrate, the NarX will change configuration and then phosphorylate. After that, the phosphorylated NarX will transport the phosphate group to NarL. Last, the phosphorylated NarL will bind to the certain sequence of PyeaR, and it acts as a transcription factor, which can active the downstream of PyeaR to transcription.
The NarX (C415R) is a mutant of NarX (BBa_K3733000), which amino acid at position of 415 changed from cysteine to arginine. For this change, we can make the nitrate sensing system more sensitive.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]