Difference between revisions of "Part:BBa K3889122"

Latest revision as of 16:27, 19 October 2021

Bacillus subtilis mf-Lon cassette

This gene cassette has been designed to produce an anti-toxin mf-Lon continuously. The modified mf-lon gene has been placed downstream to a constitutive Synthetic promoter (SP146) of Bacillus subtilis and an RBS of Bacillus subtilis (BBa_K1351030). The gene producing mf-Lon is upstream to the porcine teschovirus-1 P2A Peptide Linker (BBa_K3889069) and the reporter gene sfGFP (BBa_K3889002). This reporter will help in determining the working of the cassette in Bacillus subtilis. Finally, the cassette is completed by adding a double terminator of Bacillus subtilis (BBa_B0015) downstream to the reporter gene.

This cassette has been developed to be a part of a light-inducible kill switch in Bacillus subtilis. The constitutive production of the anti-toxin mf-Lon will aid in controlling any leaky expression of the bpDNase1 toxin by forming a complex with it. This will help in avoiding the accidental death of bacteria due to leaky expression of the toxin.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 2503

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3889122 short</partinfo>
-Tag-specific protease cassette for regulation in Bacillus subtilis.
+This gene cassette has been designed to produce an anti-toxin mf-Lon continuously. The modified mf-lon gene has been placed downstream to a constitutive Synthetic promoter (SP146) of Bacillus subtilis and an RBS of Bacillus subtilis (<partinfo>BBa_K1351030</partinfo>). The gene producing mf-Lon is upstream to the porcine teschovirus-1 P2A Peptide Linker (<partinfo>BBa_K3889069</partinfo>) and the reporter gene sfGFP (<partinfo>BBa_K3889002</partinfo>). This reporter will help in determining the working of the cassette in Bacillus subtilis. Finally, the cassette is completed by adding a double terminator of Bacillus subtilis (<partinfo>BBa_B0015</partinfo>) downstream to the reporter gene.
+This cassette has been developed to be a part of a light-inducible kill switch in Bacillus subtilis. The constitutive production of the anti-toxin mf-Lon will aid in controlling any leaky expression of the bpDNase1 toxin by forming a complex with it. This will help in avoiding the accidental death of bacteria due to leaky expression of the toxin.
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