Difference between revisions of "Part:BBa K3846017"
 
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<partinfo>BBa_K3846017 short</partinfo>
 
<partinfo>BBa_K3846017 short</partinfo>
  
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One of 2 isozymes that catalyze the conversion of HMG-CoA to mevalonate. It is the rate-limiting enzyme of the sterol biosynthesis pathway. Involved in ergosterol biosynthesis.
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This part features the codon-optimised coding sequence of a truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (HMG1). It is one of 2 isozymes that catalyze the conversion of HMG-CoA to mevalonate and the rate-limiting enzyme of the sterol biosynthesis pathway. HMG1 is also involved in ergosterol biosynthesis.
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The coding sequence of HMG1 is N-terminal truncated and contains only amino acids 520 – 1054 to exclude the complicated transmembrane tethering sequence at the start of the protein.
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The corresponding phytobrick part is [https://parts.igem.org/Part:BBa_K3846119 BBa_K3846119].
  
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520 – 1054 aa.
 
  
 
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<partinfo>BBa_K3846017 parameters</partinfo>
 
<partinfo>BBa_K3846017 parameters</partinfo>
 
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===iGEM Hamburg 2021 part collection===
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Terpenoids are an important group of natural products used as biofuels, drugs or fragrances.  Naturally occuring in plants it has been shown that microbial terpene production in microorganisms like yeast, E. coli or  cyanobacteria is possible.
 +
Nevertheless iGEM projects seem to rarely focus on this interesting class of natural products which is correlated with a lack of useful parts inside the iGEM registry.
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 +
Fortunately we were able to change that and designed a novel golden gate based toolbox which allows.
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<ol style="list-style-type:lower-alpha">
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  <li>production of terpenoid precursors and simple terpenoids</li>
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  <li>creation of CYP P450-reductase fusion enzymes to optimise processing of terpenoid precursors and production of bioactive target products</li>
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  <li>modularity of the system to enable exchange of linker sequences/promoters/etc. (MoClo-compatible toolbox)</li>
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</ol>
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===MoClo-based Part Design 2.0===
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<p>To improve the usefulness of our parts, we then aimed to make our parts compatible with the MoClo standard of goten gate based IIS restriction enzyme assembly. Thereby we expanded the Common Genetic Syntax for fusion sites to allow the creation of a) fusion proteins connected by linker sequences and b) multiple CDS expressed in an operon.
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More useful information and an overview of all our parts can be found on our [https://2021.igem.org/Team:Hamburg/Part_Collection wiki].
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[[File:T--Hamburg--parts overview MolClo.png|600px|thumb|left|'''Figure 1''': <b> MoClo syntax of the part collection. </b> <br>]]

Latest revision as of 16:21, 19 October 2021


truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (HMG1) - MevT-Pathway

This part features the codon-optimised coding sequence of a truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (HMG1). It is one of 2 isozymes that catalyze the conversion of HMG-CoA to mevalonate and the rate-limiting enzyme of the sterol biosynthesis pathway. HMG1 is also involved in ergosterol biosynthesis.

The coding sequence of HMG1 is N-terminal truncated and contains only amino acids 520 – 1054 to exclude the complicated transmembrane tethering sequence at the start of the protein.

The corresponding phytobrick part is BBa_K3846119.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



iGEM Hamburg 2021 part collection

Terpenoids are an important group of natural products used as biofuels, drugs or fragrances. Naturally occuring in plants it has been shown that microbial terpene production in microorganisms like yeast, E. coli or cyanobacteria is possible. Nevertheless iGEM projects seem to rarely focus on this interesting class of natural products which is correlated with a lack of useful parts inside the iGEM registry.

Fortunately we were able to change that and designed a novel golden gate based toolbox which allows.

  1. production of terpenoid precursors and simple terpenoids
  2. creation of CYP P450-reductase fusion enzymes to optimise processing of terpenoid precursors and production of bioactive target products
  3. modularity of the system to enable exchange of linker sequences/promoters/etc. (MoClo-compatible toolbox)

MoClo-based Part Design 2.0

To improve the usefulness of our parts, we then aimed to make our parts compatible with the MoClo standard of goten gate based IIS restriction enzyme assembly. Thereby we expanded the Common Genetic Syntax for fusion sites to allow the creation of a) fusion proteins connected by linker sequences and b) multiple CDS expressed in an operon. More useful information and an overview of all our parts can be found on our wiki.

Figure 1: MoClo syntax of the part collection.