Difference between revisions of "Part:BBa K3989004"
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Latest revision as of 16:16, 19 October 2021
EsaR D91G mutant
A variant of the Quorum Sensing regulator protein EsaR BBa_K2116001 with the Aspartic acid at position 91 substituted by Glycine.
Characterisation
According to the literature[1], the amino acid substitution has increased the sensitivity of this protein to 3OC6HSL molecule. The result of our characterisation is shown below and more details can be found in part BBa_K3989003 .
Figure 1. Fluorescence intensity measurement by plate reader(96-well plate). The measurements were done every one hour and this is the curve of the last test.
Figure 2. Fluorescence intensity measurement by flow cytometry. The samples are taken from the plate, in which the bacteria has been cultured for 7 hours.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1) Shong, J., Huang, Y. M., Bystroff, C., & Collins, C. H. (2013). Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS chemical biology, 8(4), 789-795.