Difference between revisions of "Part:BBa K3799026:Design"
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===Source=== | ===Source=== | ||
− | Csm6 is encoded by several bacteria that use Type III CRISPR-Cas systems. The sequences we use in our characterization are derived from Enterococcus italicus. | + | Csm6 is encoded by several bacteria that use Type III CRISPR-Cas systems. The sequences we use in our characterization are derived from Enterococcus italicus.<br> |
+ | https://www.uniprot.org/uniprot/E6LHV2 | ||
===References=== | ===References=== |
Revision as of 15:58, 19 October 2021
Type III-A CRISPR-Cas effector ribonuclease---Csm6
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For easy protein purification, his tag with a TEV cut site is added in the 5' end of the coding sequence.
Source
Csm6 is encoded by several bacteria that use Type III CRISPR-Cas systems. The sequences we use in our characterization are derived from Enterococcus italicus.
https://www.uniprot.org/uniprot/E6LHV2