Difference between revisions of "Part:BBa M10052"

Revision as of 14:51, 10 May 2009

{˂ehaB!}

The EhaB part codes for an autotransporter protein that can transport itself across the bacterial membrane and go to the cell surface. Like other autotransporter proteins, the EhaB part has an N-terminal signal sequence, a passenger domain, and a translocation domain. EhaB expressed in E. Coli MS427 has been shown to form biofilms on polystyrene surfaces. Also, overexpression of the protein in E. Coli K-12 has resulted in biofilm formation under continuous flow conditions.
The EhaB protein expression in E. Coli K12 and EDL933 was shown to promote adhesion to the extracellular matrix proteins collagen I and laminin. Moreover, EhaB can be expressed in vivo and can elicit an IgA immune response.

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 589
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 589
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 589
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 589
Illegal AgeI site found at 132
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_M10052 short</partinfo>
-The ehaB part codes for an autotransporter protein that is on the cell surface. The part can transport itself across the bacterial membrane and go to the cell surface. The ehaB part, like other autotransporter proteins, has an N-terminal signal sequence, a passenger domain, and a translocation domain. The ehaB part has also been shown to form biofilms on polystyrene surfaces. <br>
+The EhaB part codes for an autotransporter protein that can transport itself across the bacterial membrane and go to the cell surface. Like other autotransporter proteins, the EhaB part has an N-terminal signal sequence, a passenger domain, and a translocation domain. EhaB expressed in E. Coli MS427 has been shown to form biofilms on polystyrene surfaces. Also, overexpression of the protein in E. Coli K-12 has resulted in biofilm formation under continuous flow conditions. <br>
+The EhaB protein expression in E. Coli K12 and EDL933 was shown to promote adhesion to the extracellular matrix proteins collagen I and laminin. Moreover, EhaB can be expressed in vivo and can elicit an IgA immune response.
 ----
 This part is in BglBricks Standard.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BglBricks Standard is available at: