Difference between revisions of "Part:BBa K3787003"
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MHETase from Ideonella Sakaiensis. The sequence is optimized for the expression of Escherichia Coli, strains DH5[alpha] and C41(DE3)
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<p>A coding sequence of the MHETase from Ideonella sakaiensis. <br>
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The sequence is optimized for the expression of Escherichia coli, strains DH5α and C41(DE3). It can be added to the reaction with PETase to synergize PET degradation. It can also be used as a control to compare the hydrolytic activity of PETase-MHETase chimeric proteins in the two-enzyme system.
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Revision as of 14:46, 19 October 2021


I.s. MHETase

A coding sequence of the MHETase from Ideonella sakaiensis.
The sequence is optimized for the expression of Escherichia coli, strains DH5α and C41(DE3). It can be added to the reaction with PETase to synergize PET degradation. It can also be used as a control to compare the hydrolytic activity of PETase-MHETase chimeric proteins in the two-enzyme system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 983
    Illegal PstI site found at 1012
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 132
    Illegal PstI site found at 983
    Illegal PstI site found at 1012
    Illegal NotI site found at 680
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 983
    Illegal PstI site found at 1012
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 983
    Illegal PstI site found at 1012
    Illegal NgoMIV site found at 607
  • 1000
    COMPATIBLE WITH RFC[1000]