Difference between revisions of "Part:BBa K3740057"
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<partinfo>BBa_K3740057 short</partinfo> | <partinfo>BBa_K3740057 short</partinfo> | ||
+ | ===Description=== | ||
+ | We replaced the nuclease domain of pyocin S2 with that of colicin E3 to produce a chimeric bacteriocin termed SE. | ||
− | + | ===Sequence and Features=== | |
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<partinfo>BBa_K3740057 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3740057 SequenceAndFeatures</partinfo> | ||
Latest revision as of 14:31, 19 October 2021
pR-RBS300-SE-B1006
Description
We replaced the nuclease domain of pyocin S2 with that of colicin E3 to produce a chimeric bacteriocin termed SE.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2497
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2497
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2497
Illegal BglII site found at 2202
Illegal BamHI site found at 1774 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2497
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2497
Illegal NgoMIV site found at 1573
Illegal AgeI site found at 1663 - 1000COMPATIBLE WITH RFC[1000]