Difference between revisions of "Part:BBa K3740056"

 
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<partinfo>BBa_K3740056 short</partinfo>
 
<partinfo>BBa_K3740056 short</partinfo>
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===Description===
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We replaced the nuclease domain of pyocin S2 with that of colicin E3 to produce a chimeric bacteriocin termed SE.
  
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chimeric bacteriocin
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===Sequence and Features===
  
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K3740056 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3740056 SequenceAndFeatures</partinfo>
  

Revision as of 14:30, 19 October 2021


PA1/04/03-RBS400-SE-B1006

Description

We replaced the nuclease domain of pyocin S2 with that of colicin E3 to produce a chimeric bacteriocin termed SE.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2524
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2524
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2524
    Illegal BglII site found at 2229
    Illegal BamHI site found at 1801
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2524
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2524
    Illegal NgoMIV site found at 1600
    Illegal AgeI site found at 1690
  • 1000
    COMPATIBLE WITH RFC[1000]