Difference between revisions of "Part:BBa K3815001"
| Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3815001 short</partinfo> | <partinfo>BBa_K3815001 short</partinfo> | ||
| − | + | ==Usage and Biology== | |
| − | This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the targeted protein is CecropinA derived from a moth. This is the antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water. | + | This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the targeted protein is CecropinA derived from a <i>moth</i>. This is the antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.<br><br> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
| Line 11: | Line 11: | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3815001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3815001 SequenceAndFeatures</partinfo> | ||
| + | |||
| + | ==Purification== | ||
| + | [[File:Engineering 203 SDS-PAGE①.png|200px|thumb|right|SDS-PAGE of purified peptide]] | ||
Revision as of 14:23, 19 October 2021
CecropinA-Mxe GryA intein-PT-linker-ELK16
Usage and Biology
This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the targeted protein is CecropinA derived from a moth. This is the antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 814
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 67
Illegal AgeI site found at 346 - 1000COMPATIBLE WITH RFC[1000]
Purification
