Difference between revisions of "Part:BBa K4015000"
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4015000 parameters</partinfo>
 
<partinfo>BBa_K4015000 parameters</partinfo>
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There are mainly two types of commonly used Lcp: Lcpk30 and Lcp1 VH2. In our project, we are looking for a best Lcp considering their rubber degrading ability.  
 
There are mainly two types of commonly used Lcp: Lcpk30 and Lcp1 VH2. In our project, we are looking for a best Lcp considering their rubber degrading ability.  
  
 
Lcp was first discovered in Streptomyces sp. strain K30, so the latex clearing protein from this strain was named Lcpk30.
 
Lcp was first discovered in Streptomyces sp. strain K30, so the latex clearing protein from this strain was named Lcpk30.
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Lcp1VH2 came from Gordonia polyisoprenivorans VH2. Study has shown that Lcp1 VH2 can be grown heterogeneously in E. coli with a relatively high yield. An active degradation activity is observed in vitro experiment at the same time. In addition, Lcp1 VH2 is also remarkable for its fast metabolization. (4) Therefore, Lcp1 VH2 was included into the target protein products we plan to focus on in our experiment. 
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However, Lcp, as introduced in the articles, is flawed. The main problem of Lcp is its insolubility. Solubility is usually a great problem in most protein production, because insoluble protein will not allow any further processing of protein. As a result, the insolubility of protein will contribute to a low concentration of target protein after purification process. In order to overcome this problem a series of research and experiments were done.

Revision as of 14:21, 19 October 2021


lcp

lcp

latex clearing protein


Usage and Biology

Lcp means latex clearing protein


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

There are mainly two types of commonly used Lcp: Lcpk30 and Lcp1 VH2. In our project, we are looking for a best Lcp considering their rubber degrading ability.

Lcp was first discovered in Streptomyces sp. strain K30, so the latex clearing protein from this strain was named Lcpk30.

Lcp1VH2 came from Gordonia polyisoprenivorans VH2. Study has shown that Lcp1 VH2 can be grown heterogeneously in E. coli with a relatively high yield. An active degradation activity is observed in vitro experiment at the same time. In addition, Lcp1 VH2 is also remarkable for its fast metabolization. (4) Therefore, Lcp1 VH2 was included into the target protein products we plan to focus on in our experiment.

However, Lcp, as introduced in the articles, is flawed. The main problem of Lcp is its insolubility. Solubility is usually a great problem in most protein production, because insoluble protein will not allow any further processing of protein. As a result, the insolubility of protein will contribute to a low concentration of target protein after purification process. In order to overcome this problem a series of research and experiments were done.