Difference between revisions of "Part:BBa K3734002"
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<p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of CHREBP-LUC</p> | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of CHREBP-LUC</p> | ||
Revision as of 14:19, 19 October 2021
GIP promoter
GIP promoter is anticipated in current literature that it can only be used in several certain types of cells like intestine cells, but we proved that it can function will in 293T cells which are usually used in labs.
1.Pattern diagram
Fig.1 The model diagram of CHREBP-LUC
2.Experiments
2.1 Method
We use LUC as report gene, test its real-time luminescence value at 560nm wavelength with specific devices and detect the expression level under different glucose concentration.
2.2 Result
GIP promoter is induced by glucose and the expression level rises along with the raise of glucose concentration.
Fig.2 Changes of LUC expression over time after blue light irradiation with different glucose concentrations
3.Caution
Use medium without glucose when paving in case of the effect caused by glucose residue
Reference：
[1]Anthony T. Cheung,Bama Dayanandan,Jamie T. LewisGlucose-Dependent Insulin Release from Genetically Engineered K Cells[J].Science.2000,290:1959-1962