Difference between revisions of "Part:BBa K4061090"

 
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===Functional Parameters===
 
===Functional Parameters===
 
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Revision as of 14:03, 19 October 2021


eGFP antisense characterisation

This composite part was made to characterise the efficiency of antisense mRNA as designed in BBa_K4061041 in reduction of noise. We also regulated the antisense mRNA under a PompF promoter (BBa_K4061000) to analyse the promoter's function. Rationale being that in the condition when the OmpF promoter should be active, the antisense mRNA will be more in number, therefore reducing fluorescence intensity. This construct gave us dual information- about antisense mRNA efficiency and the promoter function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1325


Functional Parameters