Difference between revisions of "Part:BBa K3798010"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K3798010 short</partinfo>
 
<partinfo>BBa_K3798010 short</partinfo>
  
−
w
+
Silver is one of the most wasted precious metals that can be found in effluents, streams and even food. As our project is to develop a precious metal recycling platform, we chose Ag+ as the demonstration ion of this phase of the project. Aptamers are oligonucleotides that can bind targeted molecules with its secondary structures, in our project, Ag+ ion. BBa_K37980010 is a Ag+ aptamer obtained from a previous paper[1].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 12: Line 12:
 
<partinfo>BBa_K3798010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3798010 SequenceAndFeatures</partinfo>
  
 +
===Characterization===
 +
==Binding efficiency and stability==
 +
[[File:T--SHSBNU_China--silver ion 1.png|600px|thumb|center]]
 +
<p  class="figure-description"><b><center>Fig.1 Remaining Ag+ ion concentration of the solution after treated by different types of aptamers. </center></b></p >
 +
Aptamer 9 shows a binding efficacy of only 36%, and was not stable in performance. Therefore, this aptamer is discarded and was not subjected for further examination.
  
 +
===Reference===
 +
1. Saran, R., Yao, L., Hoang, P., & Liu, J. (2018). Folding of the silver aptamer in a DNAzyme probed by 2-aminopurine fluorescence. Biochimie, 145, 145-150. doi: 10.1016/j.biochi.2017.07.001
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3798010 parameters</partinfo>
 
<partinfo>BBa_K3798010 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 13:59, 19 October 2021


ssDNA, an aptamer for Ag+ ion

Silver is one of the most wasted precious metals that can be found in effluents, streams and even food. As our project is to develop a precious metal recycling platform, we chose Ag+ as the demonstration ion of this phase of the project. Aptamers are oligonucleotides that can bind targeted molecules with its secondary structures, in our project, Ag+ ion. BBa_K37980010 is a Ag+ aptamer obtained from a previous paper[1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Binding efficiency and stability

T--SHSBNU China--silver ion 1.png

Fig.1 Remaining Ag+ ion concentration of the solution after treated by different types of aptamers.

Aptamer 9 shows a binding efficacy of only 36%, and was not stable in performance. Therefore, this aptamer is discarded and was not subjected for further examination.

Reference

1. Saran, R., Yao, L., Hoang, P., & Liu, J. (2018). Folding of the silver aptamer in a DNAzyme probed by 2-aminopurine fluorescence. Biochimie, 145, 145-150. doi: 10.1016/j.biochi.2017.07.001