Difference between revisions of "Part:BBa M10019"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_M10019 short</partinfo> | <partinfo>BBa_M10019 short</partinfo> | ||
| − | Flag is a fusion protein tag. Flag is a short hydrophilic 8-amino acid peptide that binds to the antibody M1. The flag-tag can be at either the N or C terminus of the protein of interest. | + | Flag is a fusion protein tag. Flag is a short hydrophilic 8-amino acid peptide that binds to the antibody M1. The flag-tag can be at either the N or C terminus of the protein of interest. When purifying, the purification condition of the system is non-denaturing which means it allows for active fusion proteins to be purified. |
PMID: 12536251 | PMID: 12536251 | ||
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Revision as of 04:17, 10 May 2009
{˂FLAG˃} Flag is a fusion protein tag. Flag is a short hydrophilic 8-amino acid peptide that binds to the antibody M1. The flag-tag can be at either the N or C terminus of the protein of interest. When purifying, the purification condition of the system is non-denaturing which means it allows for active fusion proteins to be purified. PMID: 12536251
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]