Difference between revisions of "Part:BBa M10018"

Revision as of 03:46, 10 May 2009

{˂Z34C domain˃} Protein A is a protein found in Staphylococcus aureus. The protein is good at binding to IgG antibodies There's two sites on IgG that it can bind to. The fab and the fc portions. The fab is of the the 2 heads of the y antibody and the fc is the (constant region) tail. Since we're only interesting in the tail, an effort was made to find the smallest protein domain possible that still binds to the fc portion of IgG. They were using phages to select for smaller proteins which still bind. Thus, they first came up with the Z38 protein. However, Z38 turned out to be unstable which lead to the development of Z34C (from furthermore phage display) which actually bind much tighter than Z38 to fc, but loses the ability to bind to fab. PMID: 9294166

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Sequence and Features