Difference between revisions of "Part:BBa K3805283:Design"

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===Design Notes===
 
===Design Notes===
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This part is designed to guide dCas9 to silence the function of the promoter pBAD and to inhibit the initiation of downstream genes.In our project, we used this part to suppress the activation of the kill system.The suppression caused by dCas9 is reversible and does not permanently alter the genomic DNA.This sequence is designed and evaluated by the website http://crispr.dfci.harvard.edu/SSC/.
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In order to ensure the work efficiency, the length of sgRNA should be controlled at 19-20bp, and the selection of binding sequence should be specific.
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===Source===
 
===Source===

Latest revision as of 13:42, 19 October 2021


sgRNA bind to Pbad promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to ensure the work efficiency, the length of sgRNA should be controlled at 19-20bp, and the selection of binding sequence should be specific.

Source

We expect to synthesize this fragment.

References