Difference between revisions of "Part:BBa M10025"

Revision as of 02:39, 10 May 2009

{˂GFP-LVA˃} Fast-Degrading GFP

The purpose of this part is to encode a fast degrading GFP. Wildtype GFP typically have a half-life that may last for several bacterial generations. However, the addition of an AANDENYALAA tag to the C-terminal of the wildtype GFP will decrease this halflife to 40 minutes (Andersen et al, 1998). By changing the last three residues of this tag, various fast-degrading GFP were created. GFP[LVA] has a halflife of 40 minutes (Miller et al., 2000).

Usage of a fast degrading GFP may act as a better reporter of changes in gene transcription over a period of time.

PMID: 11059491

This part follows the BglBricks standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Format]

Sequence and Features