Difference between revisions of "Part:BBa K3798004"
 
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===Reference===
 
===Reference===
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1. Hall Sedlak, R., Hnilova, M., Grosh, C., Fong, H., Baneyx, F., & Schwartz, D. et al. (2012). Engineered Escherichia coli Silver-Binding Periplasmic Protein That Promotes Silver Tolerance. Applied And Environmental Microbiology, 78(7), 2289-2296. doi: 10.1128/aem.06823-11
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1. Shen, F., Mao, S., Mathivanan, J., Wu, Y., Chandrasekaran, A., & Liu, H. et al. (2020). Short DNA Oligonucleotide as a Ag+ Binding Detector. ACS Omega, 5(44), 28565-28570. doi: 10.1021/acsomega.0c03372
  
  

Latest revision as of 13:37, 19 October 2021


ssDNA, a good aptamer for Ag+ ion 1

Silver is one of the most wasted precious metals that can be found in effluents, streams and even food. As our project is to develop a precious metal recycling platform, we chose Ag+ as the demonstration ion of this phase of the project. Aptamers are oligonucleotides that can bind targeted molecules with its secondary structures, in our project, Ag+ ion. BBa_K3798004 is a Ag+ aptamer obtained from a previous paper[1]. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Binding efficiency and stability

T--SHSBNU China--silver ion 1.png

Fig.1 Remaining Ag+ ion concentration of the solution after treated by different types of aptamers.

Although Aptamer 3 has the highest binding ability (the remaining ion concentration of Aptamer 3 is 86ug/L lower than that of Aptamer 4), the performance of it is not as stable as that of Aptamer 4 (larger error bound) and cannot be used in large scale implementation. Therefore, further inspection of this aptamer did not proceed.


Reference

1. Shen, F., Mao, S., Mathivanan, J., Wu, Y., Chandrasekaran, A., & Liu, H. et al. (2020). Short DNA Oligonucleotide as a Ag+ Binding Detector. ACS Omega, 5(44), 28565-28570. doi: 10.1021/acsomega.0c03372