Difference between revisions of "Part:BBa K3798002"

Revision as of 13:35, 19 October 2021

ssDNA, an aptamer for Ag+ ion

Silver is one of the most wasted precious metals that can be found in effluents, streams and even food. As our project is to develop a precious metal recycling platform, we chose Ag+ as the demonstration ion of this phase of the project. Aptamers are oligonucleotides that can bind targeted molecules with its secondary structures, in our project, Ag+ ion. BBa_K3798002 is a Ag+ aptamer obtained from a previous paper[1].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Characterization

Binding efficiency and stability

Fig.1 Remaining Ag+ ion concentration of the solution after treated by different types of aptamers.

Aptamer 1 shows a binding efficacy of about 75%, although it seems like a high binding efficiency, it do not satisfies the safe limit of concentration of the silver ions. Therefore, this aptamer is discarded and was subjected for further examination.

Reference

1. Zhou, X., Memon, A., Sun, W., Fang, F., & Guo, J. (2020). Fluorescent Probe for Ag+ Detection Using SYBR GREEN I and C-C Mismatch. Biosensors, 11(1), 6. doi: 10.3390/bios11010006

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 ===Reference===
-. Hall Sedlak, R., Hnilova, M., Grosh, C., Fong, H., Baneyx, F., & Schwartz, D. et al. (2012). Engineered Escherichia coli Silver-Binding Periplasmic Protein That Promotes Silver Tolerance. Applied And Environmental Microbiology, 78(7), 2289-2296. doi: 10.1128/aem.06823-11
+. Zhou, X., Memon, A., Sun, W., Fang, F., & Guo, J. (2020). Fluorescent Probe for Ag+ Detection Using SYBR GREEN I and C-C Mismatch. Biosensors, 11(1), 6. doi: 10.3390/bios11010006
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