Difference between revisions of "Part:BBa M10018:Design"

Latest revision as of 02:30, 10 May 2009

{˂Z34C domain˃}

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

later

Source

Synthetic

Wobble Jw0017F / Jw0018R (133bp, EcoRI/BamHI)
Sub into pBca9145-Bca1144#5 (EcoRI/BamHI, 2057+910, L)
Product is pBca9145-M10018 {Z34C}

Jw0017F Forward construction of Z34C
CCATAgaattcATGagatctTTCAACATGCAGTGCCAGCGTCGTTTCTACGAAGCTCTGCACGACCCGAACCTGAACGAAG
Jw0018R Reverse construction of Z34C
CTGATggatccGCAGTCGTCACGGATAGATTTGATTTTAGCGTTACGCTGTTCTTCGTTCAGGTTCGGGTCG

References

Starovasnik MA, Braisted AC, Wells JA. (1997) Structural mimicry of a native protein by a minimized binding domain. Proceedings National Academy of Science USA. 94:10080-5.

Revision as of 02:18, 10 May 2009 (view source) Johnwang (Talk \| contribs) (→‎Source) ← Older edit		Latest revision as of 02:30, 10 May 2009 (view source) Johnwang (Talk \| contribs) (→‎References)
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	===References===		===References===
		+	Starovasnik MA, Braisted AC, Wells JA. (1997) Structural mimicry of a native protein by a minimized binding domain. Proceedings National Academy of Science USA. 94:10080-5.