Difference between revisions of "Part:BBa K3734002"

 
Line 7: Line 7:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
<h2 class="pageContent-main__title">
 +
                    <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title-->
 +
                    GIP promoter
 +
                </h2>
 +
                <div class="pageContent-main__textBox">
 +
                    <!--all the content must included in this div-->
 +
                    <p>GIP promoter is anticipated in current literature that it can only be used in several certain types of cells like intestine cells, but we proved that it can function will in 293T cells which are usually used in labs.
 +
                    </p>
 +
                </div>
 +
                <h2 class="pageContent-main__title pageContent-main__subtitle">
 +
                    <!--class="pageContent-main__title" means it is the sub title-->
 +
                    1.Pattern diagram
 +
                </h2>
 +
                <div class="pageContent-main__textBox">
 +
                    <!--all the content must included in this div-->
 +
                    <img class="image" src="https://2021.igem.org/wiki/images/8/87/T--CSU_CHINA--tupian5.png">
 +
                    <!--put image's url here-->
 +
                    <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of CHREBP-LUC</p>
 +
                </div>
 +
                <h2 class="pageContent-main__title pageContent-main__subtitle">
 +
                    <!--class="pageContent-main__title" means it is the sub title-->
 +
                    2.Experiments
 +
                </h2>
 +
                <h2 class="pageContent-main__title pageContent-main__subtitle">
 +
                    <!--class="pageContent-main__title" means it is the sub title-->
 +
                    2.1 Method
 +
                </h2>
 +
                <div class="pageContent-main__textBox">
 +
                    <!--all the content must included in this div-->
 +
                    <p>We use LUC as report gene, test its real-time luminescence value at 560nm wavelength with specific devices and detect the expression level under different glucose concentration.
 +
                    </p>
 +
                    <!--put text here, <p>content</p>-->
 +
                </div>
 +
                <h2 class="pageContent-main__title pageContent-main__subtitle">
 +
                    <!--class="pageContent-main__title" means it is the sub title-->
 +
                    2.2 Result
 +
                </h2>
 +
                <div class="pageContent-main__textBox">
 +
                    <!--all the content must included in this div-->
 +
                    <p>GIP promoter is induced by glucose and the expression level rises along with the raise of glucose concentration.
 +
                    </p>
 +
                    <img class="image" src="https://2021.igem.org/wiki/images/5/59/T--CSU_CHINA--tupian6.png">
 +
                    <!--put image's url here-->
 +
                    <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Changes of LUC expression over time after blue light irradiation with different glucose concentrations</p>
 +
                    <!--put text here, <p>content</p>-->
 +
                </div>
 +
                <h2 class="pageContent-main__title pageContent-main__subtitle">
 +
                    <!--class="pageContent-main__title" means it is the sub title-->
 +
                    3.Caution
 +
                </h2>
 +
                <div class="pageContent-main__textBox">
 +
                    <!--all the content must included in this div-->
 +
                    <p>Use medium without glucose when paving in case of the effect caused by glucose residue
 +
                    </p>
 +
                </div>
 +
                <h2 class="pageContent-main__title pageContent-main__subtitle">
 +
                    <!--class="pageContent-main__title" means it is the sub title-->
 +
                    Reference：
 +
                </h2>
 +
                <div class="pageContent-main__textBox">
 +
                    <!--all the content must included in this div-->
 +
                    <p>[1]Anthony T. Cheung,Bama Dayanandan,Jamie T. LewisGlucose-Dependent Insulin Release from Genetically Engineered K Cells[J].Science.2000,290:1959-1962
 +
                    </p>
 +
                </div>
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 13:18, 19 October 2021


GIP promoter

GIP is a promoter that can be induced by glucose. 

                   GIP promoter
               </h2>

GIP promoter is anticipated in current literature that it can only be used in several certain types of cells like intestine cells, but we proved that it can function will in 293T cells which are usually used in labs.

1.Pattern diagram

                   <img class="image" src="T--CSU_CHINA--tupian5.png">

Fig.1 The model diagram of CHREBP-LUC

2.Experiments

2.1 Method

We use LUC as report gene, test its real-time luminescence value at 560nm wavelength with specific devices and detect the expression level under different glucose concentration.

2.2 Result

GIP promoter is induced by glucose and the expression level rises along with the raise of glucose concentration. 

                   <img class="image" src="T--CSU_CHINA--tupian6.png">

Fig.2 Changes of LUC expression over time after blue light irradiation with different glucose concentrations

3.Caution

Use medium without glucose when paving in case of the effect caused by glucose residue

Reference：

[1]Anthony T. Cheung,Bama Dayanandan,Jamie T. LewisGlucose-Dependent Insulin Release from Genetically Engineered K Cells[J].Science.2000,290:1959-1962

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 899
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216