Difference between revisions of "Part:BBa M10025:Design"

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===References===
 
===References===
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Andersen JB et al (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. ''Appl. Environ. Microbiol.'' 64: 2240-2246.
+
Andersen JB et al (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. ''Appl. Environ. Microbiol.'' 64: 2240-2246.<br>
 
Miller WG et al (2000). Improved gfp and inaZ broad-host-range promoter-probe vectors. ''Mol Plant Microbe Interact.'' 13(11): 1243-1250.
 
Miller WG et al (2000). Improved gfp and inaZ broad-host-range promoter-probe vectors. ''Mol Plant Microbe Interact.'' 13(11): 1243-1250.

Revision as of 01:32, 10 May 2009

{˂GFP-LVA˃} Fast-Degrading GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 716
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 716
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 716
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 716
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641


Design Notes

This part follows the BglBricks standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Format]


This part was made using a LR Gateway Transfer reaction. More information on this process can be found here:
[http://2008.igem.org/Team:UC_Berkeley/GatewayOverview LR Gateway Transfer Overview]

The donor plasmid pBca1256-Bjh1857, which contains the gene GFP-LVA, underwent a Gateway Transfer reaction with the recipient plasmid pBca1254AK.

Source

pBca1256-Bjh1857

References

Andersen JB et al (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64: 2240-2246.
Miller WG et al (2000). Improved gfp and inaZ broad-host-range promoter-probe vectors. Mol Plant Microbe Interact. 13(11): 1243-1250.