Difference between revisions of "Part:BBa K3765020:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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None.
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This protein generator contains SpeI and XbaI restriction sites from the vector pETMCN. We used Gibson Assembly for cloning, so there were no hindrances in this regard. In case someone wants to use this part, it is suggested to get it synthesized and use Gibson Assembly (or other such assembly techniques) for cloning.
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===Source===
 
===Source===

Latest revision as of 12:52, 19 October 2021


SpyCatcher002+dCBD under T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 981
    Illegal SpeI site found at 45
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 987
    Illegal SpeI site found at 45
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 723
    Illegal BamHI site found at 969
    Illegal XhoI site found at 365
    Illegal XhoI site found at 951
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 981
    Illegal SpeI site found at 45
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 981
    Illegal SpeI site found at 45
    Illegal AgeI site found at 195
    Illegal AgeI site found at 561
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 450


Design Notes

This protein generator contains SpeI and XbaI restriction sites from the vector pETMCN. We used Gibson Assembly for cloning, so there were no hindrances in this regard. In case someone wants to use this part, it is suggested to get it synthesized and use Gibson Assembly (or other such assembly techniques) for cloning.

Source

Synthetic.

References