Difference between revisions of "Part:BBa M10025:Design"
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Revision as of 00:55, 10 May 2009
{˂GFP-LVA˃} Fast-Degrading GFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 716
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 716
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 716
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 716
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 641
Design Notes
This part follows the BglBricks standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Format]
This part was made using a LR Gateway Transfer reaction. More information on this process can be found here:
[http://2008.igem.org/Team:UC_Berkeley/GatewayOverview LR Gateway Transfer Overview]
Source
pBca1256-Bjh1857