Difference between revisions of "Part:BBa K2012015:Experience"
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<p class="MsoNormal"><strong>Fig.1 <a name="OLE_LINK16" id="OLE_LINK16"></a><a name="OLE_LINK15" id="OLE_LINK15">Mean  sfGFP fluorescence of CcaS-CcaR</a> system under green light, red light  and darkness.</strong></p>
 
<p class="MsoNormal"><strong>Fig.1 <a name="OLE_LINK16" id="OLE_LINK16"></a><a name="OLE_LINK15" id="OLE_LINK15">Mean  sfGFP fluorescence of CcaS-CcaR</a> system under green light, red light  and darkness.</strong></p>
 
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<p class="MsoNormal">As is shown in the figure, in E.coli strain  JT2, PcpcG2 produces 266.0 &plusmn; 19.3 and 509.0 &plusmn; 55.4 au of sfGFP in red and green  light, corresponding to 1.91 &plusmn; 0.34-fold activation, demonstrating that PcpcG2  is functionally regulated by light, green-activated and red-repressed.</p>
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<p class="MsoNormal">However, the figure also shows that that  leaked expression is severe in dark surroundings. To better respond to red and  green light, we optimized the promoter by refactoring it and created an  innovative promoter, PcpcG2-172, with the truncation of a constitutive promoter  within the cpcG2 promoter (BBa_K2012015. For more detail, please view our wiki:  http://2016.igem.org/Team:HZAU-China/Experiments). </p>
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Revision as of 12:11, 19 October 2021


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Experience

 

Promoter cpcG2 is a 238bp green-light activated promoter from the genome of Synechocystis PCC6803. We tested the efficiency of the promoter by measuring the fluorescence of output sfGFP when bacteria are illuminated with green, red or no light. (For more detail, please view our wiki: http://2016.igem.org/Team:HZAU-China/Experiments)

 

00.1

Fig.1 Mean sfGFP fluorescence of CcaS-CcaR system under green light, red light and darkness.

 

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