Difference between revisions of "Part:BBa K4083008"
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[1] Tiso, T., Sabelhaus, P., Behrens, B., Wittgens, A., Rosenau, F., Hayen, H., & Blank, L. M. (2016b). Creating metabolic demand as an engineering strategy in Pseudomonas putida – Rhamnolipid synthesis as an example. Metabolic Engineering Communications, 3, 234–244. https://doi.org/10.1016/j.meteno.2016.08.002
 
[1] Tiso, T., Sabelhaus, P., Behrens, B., Wittgens, A., Rosenau, F., Hayen, H., & Blank, L. M. (2016b). Creating metabolic demand as an engineering strategy in Pseudomonas putida – Rhamnolipid synthesis as an example. Metabolic Engineering Communications, 3, 234–244. https://doi.org/10.1016/j.meteno.2016.08.002
  

Revision as of 12:00, 19 October 2021


RPGDuo2 plasmid with nadE and rhlBA genes

This plasmid consists of four parts: RPGDuo2 plasmid; nadE, rhlA, and rhlB coding sequences (https://parts.igem.org/Part:BBa_K4083000, https://parts.igem.org/Part:BBa_K4083004, https://parts.igem.org/Part:BBa_K4083006, https://parts.igem.org/Part:BBa_K4083007).


By inserting this plasmid, we predicted the effective rhamnolipid production by Pseudomonas putida. Since Pseudamonas putida has all genes required for rhamnolid production except for rhlAB, the rhlA and rhlb coding sequnences inserted in plasmid can allow engineered P. putida to synthesize mono-rhamnolipid by itself. [1]

Reference:

[1] Tiso, T., Sabelhaus, P., Behrens, B., Wittgens, A., Rosenau, F., Hayen, H., & Blank, L. M. (2016b). Creating metabolic demand as an engineering strategy in Pseudomonas putida – Rhamnolipid synthesis as an example. Metabolic Engineering Communications, 3, 234–244. https://doi.org/10.1016/j.meteno.2016.08.002

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5565
    Illegal BamHI site found at 6125
    Illegal XhoI site found at 1757
    Illegal XhoI site found at 2024
    Illegal XhoI site found at 6301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1949
    Illegal NgoMIV site found at 2019
    Illegal NgoMIV site found at 2240
    Illegal NgoMIV site found at 4400
  • 1000
    COMPATIBLE WITH RFC[1000]