Difference between revisions of "Part:BBa K4040022"
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This part is part of our design of IL-6 complex receptor. We connect TCS(<partinfo>BBa_J18918</partinfo>) behind mil-6R(<partinfo>BBa_K4040013</partinfo>) and connect part tTA (<partinfo>BBa_K4040011</partinfo>) downstream of TCS. We used pCMV(<partinfo>BBa_I712004</partinfo>) as the promoter. When IL-6 elevates to a certain level in vivo, mIL-6R forms a hexamer complex with GP130 and IL-6 from <partinfo>BBa_K4040020</partinfo>. TEV protease in <partinfo>BBa_K2446037</partinfo> is activated. Then it binds to TCS connected to the downstream of IL-6R and digests the binding site.  TTA (<partinfo>BBa_K4040011</partinfo>) is released, which modulates the expression of another composite part we designed, BBa_K4040024, and promotes the transformation of macrophages to M2 state by promoting the expression of CAR-MERTK.
 
This part is part of our design of IL-6 complex receptor. We connect TCS(<partinfo>BBa_J18918</partinfo>) behind mil-6R(<partinfo>BBa_K4040013</partinfo>) and connect part tTA (<partinfo>BBa_K4040011</partinfo>) downstream of TCS. We used pCMV(<partinfo>BBa_I712004</partinfo>) as the promoter. When IL-6 elevates to a certain level in vivo, mIL-6R forms a hexamer complex with GP130 and IL-6 from <partinfo>BBa_K4040020</partinfo>. TEV protease in <partinfo>BBa_K2446037</partinfo> is activated. Then it binds to TCS connected to the downstream of IL-6R and digests the binding site.  TTA (<partinfo>BBa_K4040011</partinfo>) is released, which modulates the expression of another composite part we designed, BBa_K4040024, and promotes the transformation of macrophages to M2 state by promoting the expression of CAR-MERTK.
 
[[File:T--NMU China--IL6R-TCS-tTA.jpg|300px|thumb|left|<b>Figure1.</b>Structure of IL6R-TCS-tTA.]]
 
[[File:T--NMU China--IL6R-TCS-tTA.jpg|300px|thumb|left|<b>Figure1.</b>Structure of IL6R-TCS-tTA.]]
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[[File:T--NMU China--IL-6R.jpg|550px|thumb|right|<b>Figure. 2</b>The structure and downstream pathway of the composite IL6R.]]
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[[File:T--NMU China--IL-6R.jpg|550px|thumb|none|<b>Figure. 2</b>The structure and downstream pathway of the composite IL6R.]]
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===Experimental result===
 +
It can be found in <partinfo>BBa_K4040020</partinfo>.
 
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Latest revision as of 11:52, 19 October 2021


Synthetic Receptor IL6R-TCS-tTA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2960
    Illegal BamHI site found at 1876
    Illegal BamHI site found at 3155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 142
    Illegal NgoMIV site found at 166
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 250
    Illegal NgoMIV site found at 2515
    Illegal NgoMIV site found at 2578
    Illegal NgoMIV site found at 2947
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1972
    Illegal BsaI.rc site found at 3055

Usage and Biology

This part is part of our design of IL-6 complex receptor. We connect TCS(BBa_J18918) behind mil-6R(BBa_K4040013) and connect part tTA (BBa_K4040011) downstream of TCS. We used pCMV(BBa_I712004) as the promoter. When IL-6 elevates to a certain level in vivo, mIL-6R forms a hexamer complex with GP130 and IL-6 from BBa_K4040020. TEV protease in BBa_K2446037 is activated. Then it binds to TCS connected to the downstream of IL-6R and digests the binding site.  TTA (BBa_K4040011) is released, which modulates the expression of another composite part we designed, BBa_K4040024, and promotes the transformation of macrophages to M2 state by promoting the expression of CAR-MERTK.

Figure1.Structure of IL6R-TCS-tTA.
Figure. 2The structure and downstream pathway of the composite IL6R.

Experimental result

It can be found in BBa_K4040020.