Difference between revisions of "Part:BBa K3796005"
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The RBS sequence, or ribosome binding site, is located several sequences upstream of the gene expression frame and is an important sequence for pre-translational recognition and binding of the ribosome. When the DNA fragment downstream of the promoter and upstream of the terminator is transcribed out of the mRNA, the ribosome recognizes and binds the RBS sequence on the mRNA and then scans down until it recognizes the start codon and begins translation. | The RBS sequence, or ribosome binding site, is located several sequences upstream of the gene expression frame and is an important sequence for pre-translational recognition and binding of the ribosome. When the DNA fragment downstream of the promoter and upstream of the terminator is transcribed out of the mRNA, the ribosome recognizes and binds the RBS sequence on the mRNA and then scans down until it recognizes the start codon and begins translation. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here--> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | This is a weak strength RBS, which is optimized from the original ones in Corynebacterium glutamicum and can decreasing gene expression by reducing translation in Corynebacterium glutamicum. | |
| + | <br> | ||
| + | By applying the weak strength RBS, reducing gene expression be made through regulation on the translation level. It has been verified that this RBS can realize reduction in Corynebacterium glutamicum. However, application in other species is not clear yet. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 11:39, 19 October 2021
Corynebacterium glutamicum weak RBS
The RBS sequence, or ribosome binding site, is located several sequences upstream of the gene expression frame and is an important sequence for pre-translational recognition and binding of the ribosome. When the DNA fragment downstream of the promoter and upstream of the terminator is transcribed out of the mRNA, the ribosome recognizes and binds the RBS sequence on the mRNA and then scans down until it recognizes the start codon and begins translation.
Usage and Biology
This is a weak strength RBS, which is optimized from the original ones in Corynebacterium glutamicum and can decreasing gene expression by reducing translation in Corynebacterium glutamicum.
By applying the weak strength RBS, reducing gene expression be made through regulation on the translation level. It has been verified that this RBS can realize reduction in Corynebacterium glutamicum. However, application in other species is not clear yet.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]