Difference between revisions of "Part:BBa K3994011"

Revision as of 11:23, 19 October 2021

PyeaR_HrpR+PttB344_HrpS_PJ23105_ttrR+PhrpL_amilGFP_IL10_PJ23104_ttrS

promoter

Profile

Name: PyeaR_HrpR+PttB344_HrpS_PJ23105_ttrR+PhrpL_amilGFP_IL10_PJ23104_ttrS

Base Pairs: 7494 bp

Origin: Synthetic

Properties: A biosensor releasing fluorescence only when detecting the IBD marker.

Usage and Biology

Background

Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory disease of unknown etiology, including ulcerative colitis (CD) and Crohn’s disease (CD). This chronic disease, which is prone to repeated deterioration, currently lacks unified diagnostic and treatment standards, and is posing a great threat to public health. Drug therapy (anti-inflammatory drugs) is the preferred treatment for IBD. However, studies in the past 10 years have found that 30-50% of IBD patients do not respond to anti-TNF treatment. In addition, after long-term use of anti-inflammatory drugs, the patient's intestinal microbial status changes over time, and the effect may be lost due to drug resistance. Therefore, we need to seek help from other treatments for IBD.

Construct design

We can build Boolean logic circuits to combine thiosulfate/tetrasulfate and nitrate biosensors to optimize performance, improve sensor accuracy and enhance robustness, and optimize intestinal inflammation detection (Figure 1). When and only when S2O32-/S4O62- and NO3- are present at the same time, the fluorescent gene is expressed (Figure 1). The fluorescent gene can be replaced with a gene that produce the interleukin-10 (anti-inflammatory factor) for the treatment of IBD. HrpS is a key functional factor under PttB344 promoter. amilGFP is a fluorescent protein under PhrpL promoter. IL10 is an anti-inflammatory factor.

Figure 1. Boolean gate logic circuit/AND circuit construction...

 Part1: PyeaR_HrpR, sensing NO3- then release the substance R;

 Part2: PttB344_HrpS_PJ23105_ttrR, sensing S4O62- then release the substance S;

 Part3: PhrpL_amilGFP_IL10_PJ23104_ttrS, will release fluorescence if sensing both the substances, R and S released from Part 1(HrpR) and Part 2 (HrpS) , respectively.

Sequence and Features