Difference between revisions of "Part:BBa K3941006"

Revision as of 11:17, 19 October 2021

pET29b+EGII (C99V)

BBa_K3941006 is a composite part that we used in our expression plasmids (pET29b). We obtain EG2 wild type with this sequence.

This composite part contains 4 parts:

- T7 Promoter (BBa_K3941000)
- LacO (BBa_K1624002)
- EGII (C99V) (BBa_K3941002)
- T7 Terminator

Figure 1: T7 promoter, Lac Operon, Codon optimized EGII, 6XHis and terminator

Codon Optimized EGII

This part is created based on only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag was added to 3' end of the sequence to help purification of protein product. A single aminoacid mutation has been studied as Aspartic acid aminoacid in 185 aminoacid position is changed with Serine aminoacid.

Sequence and Features