Difference between revisions of "Part:BBa K2927006"
Line 4: Line 4:
  
 
The CRISPR/Case system uses guide RNA (gRNA) and crRNAs to recognize their targets. The gRNA sequence binds to Cas12a protein, which promotes crRNA to recognize its target double-stranded DNA through sequence complementation.
 
The CRISPR/Case system uses guide RNA (gRNA) and crRNAs to recognize their targets. The gRNA sequence binds to Cas12a protein, which promotes crRNA to recognize its target double-stranded DNA through sequence complementation.
 +
 +
<b><font size="+1.2"> Characterization by iGEM21_GreatBay_SZ </font></b>
 +
 +
*'''Group:''' iGEM21_GreatBay_SZ
 +
*'''Author:''' TianYi Huang
 +
 +
We attached the repeat sequence to guide RNA used to recognize their targets([[Part:BBa_K3859013]]). The crRNA will bind with cas12a enzyme, then the target DNA will be recobgnised and unwound by cas12a. After target DNA binds with guide RNA, cas12a will be activated and cleveage the sequence around it.
 +
In our project, we primarily used Cas12a detection technology for the identification of spores. This decision was made with reference to the methodology used in the HOLMES system
 
   
 
   
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:32, 19 October 2021


guide RNA of LbCas12a

The CRISPR/Case system uses guide RNA (gRNA) and crRNAs to recognize their targets. The gRNA sequence binds to Cas12a protein, which promotes crRNA to recognize its target double-stranded DNA through sequence complementation.

Characterization by iGEM21_GreatBay_SZ

  • Group: iGEM21_GreatBay_SZ
  • Author: TianYi Huang

We attached the repeat sequence to guide RNA used to recognize their targets(Part:BBa_K3859013). The crRNA will bind with cas12a enzyme, then the target DNA will be recobgnised and unwound by cas12a. After target DNA binds with guide RNA, cas12a will be activated and cleveage the sequence around it. In our project, we primarily used Cas12a detection technology for the identification of spores. This decision was made with reference to the methodology used in the HOLMES system

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]